Methods for studying iron metabolism in yeast mitochondria.
نویسندگان
چکیده
( B. D etermination of Cellular Heme Levels by Radiolabeling of Yeast Cells In Vivo C. D etermination of Fe/S Cluster Formation in Isolated Mitochondria In Vitro D. A nalysis of Fe/S Cluster Formation In Vitro Using Recombinant Ferredoxins III. D etermination of Mitochondrial Iron
منابع مشابه
Iron Accumulated in Mitochondria of a YFH1 - Mutant of the Yeast Saccharomyces cerevisiae Corresponds to Inorganic Ferric Phosphate
The YFH1 gene is the yeast homologue of the human FRDA gene encoding a protein named frataxin. Mutations of the frataxin gene lead to a decreased frataxin expression causing Friedreich’s ataxia, the most common autosomal recessive neurodegenerative disease of Caucasians [1,2]. A defect in the yeast frataxin homologue leads to several S. cerevisiae phenotypes. Iron uptake is considerably higher ...
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Background: Chrome is regarded as an essential nutrient required for glucose and lipid metabolism, specifically in diabetic patients. Converting mineral form of chromium to organic as well as increasing its absorption seem to have more fruitful therapeutic effects due to the synergistic effect of these two anti-diabetes compounds(yeast and chromium). Therefore, the present study aimed to evalu...
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Atm1p, a mitochondrial half-type ATP-binding cassette (ABC) protein in Saccharomyces cerevisiae, transports a precursor of the iron-sulfur (Fe/S) cluster from mitochondria to the cytosol. We have identified a novel half-type human ABC protein, designating it MTABC3 (mammalian mitochondrial ABC protein 3). MTABC3 mRNA is ubiquitously expressed in all of the rat and human tissues examined. MTABC3...
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عنوان ژورنال:
- Methods in cell biology
دوره 80 شماره
صفحات -
تاریخ انتشار 2007